1. Home page
  2. Search

Mutational activation of K-ras oncogene in human breast tumors

This item is provided by the institution :
National Hellenic Research Foundation (NHRF)   

Repository :
Helios - Repository of the National Hellenic Research Foundation (NHRF)  | repositories EKT   

see the original item page
in the repository's web site and access all digital files if the item*



Mutational activation of K-ras oncogene in human breast tumors
Malamoumitsi, V.
Σπαντίδος, Δημήτριος Α.
Koffa, M.
Agnantis, N. J.
Άρθρο σε επιστημονικό περιοδικό
1994
ras genes are thought to play an important role in human cancer since they have been found to be activated frequently in several types of human tumors. From preliminary studies it has been found however, that ras mutations are extremely rare in breast tumors and therefore it was of interest to examine the frequency of such mutations. In this study we examined 65 cases of primary breast carcinomas from paraffin blocks, for the presence of point mutations in codons 12 of the K-ras and Η-ras genes. The polymerase chain reaction (PCR) technique was used to amplify a codon 12 containing 157 bp and a 312 bp region of the K-ras and the Η-ras genes respectively, followed by restriction fragment length polymorphism (RFLP) analysis to identify the point mutations. Eight out of the 65 tumors (12.3%) were found to carry a K-ras mutation in codon 12 but none was found to carry a Η-ras mutation. It is suggested that the mutational activation of the K-ras gene may be involved in the development of a small percentage of breast tumors.
English
Demetrios A. Spandidos Ed. & Pub.
International Journal of Oncology
https://rightsstatements.org/page/InC/1.0/?language=en
© 1994 Spandidos Publications Ltd. "Reproduced by permission of the Spandidos Publications Ltd"
© 1994 Spandidos Publications Ltd. "Reproduced by permission of the Spandidos Publications Ltd" (EN)
National Hellenic Research Foundation (NHRF)
Helios - Repository of the National Hellenic Research Foundation (NHRF)



*Institutions are responsible for keeping their URLs functional (digital file, item page in repository site)