An in vitro study, evaluating the effect of sunitinib and/or lapatinib on two glioma cell lines

Argyriou, Andreas

Kalofonos, Haralabos

Giannopoulou, Efstathia

Dimitropoulos, Konstantinos

Dimitrakopoulos, Fotinos

Koutras, Angelos

Καλόφωνος, Χαράλαμπος

Δημητρόπουλος, Κωνσταντίνος

Κούτρας, Άγγελος

Γιαννοπούλου, Ευσταθία

Δημητρακόπουλος, Φωτεινός

Αργυρίου, Ανδρέας

Aim: We sought to assess the effect of sunitinib and lapatinib applied either alone or in combination, on U87 and M059K glioma cells. Methods: Both cell lines were cultured as recommended by the manufacturer. Sunitinib and lapatinib were applied, either separately or in combination, in the cultured cells after cell attachment at doses of 10nM, 100nM, 1μM and 10 μM. To determine whether the agents affect the proliferation of glioma cells, the 3-[4,5-dimethylthiazol-2-yl]-2,5 dimethyltetrazolium bromide assay was used. Apoptosis was detected using annexin V/propidium iodide detection assay, migration assay was performed in 24-well microchemotaxis chambers. The release of MMPs into the culture medium of U87 and M059K cells was measured by zymography. Results: Both agents, administered either alone or in combination, decreased cell proliferation in a dose-dependent manner 48 h after their application in both cell lines. The inhibition of their combination was statistically different than the inhibition of each agent alone. Apoptosis was increased and migration of U87 and M059K cells was inhibited either by each agent alone or their combination. MMPs levels remained unaffected by the application of both agents in U87 cells. However, MMP-9 and MMP-2 levels were decreased 48h after treatment of M059K cells with sunitinib either alone or in combination with lapatinib. Conclusion: Sunitinib and/or lapatinib appear to exhibit significant effects on proliferation, apoptosis and migration of glioma cells. When applied alone, sunitinib appears to be a more potent inhibitor than lapatinib.

Journal (paper)

Malignant glioma

Sunitinib

Migration

MMPs

Κακοήθες γλοίωμα

Lapatinib

Proliferation

Μετανάστευση

Απόπτωση

Apoptosis

Πολλαπλασιασμός

University of Patras

Nemertes

Investigational New Drugs

English

2013-12-03T10:43:48Z

2010-10

2013-12-03

http://hdl.handle.net/10889/6458

Giannopoulou E, Dimitropoulos K, Argyriou AA, Koutras AK, Dimitrakopoulos F, Kalofonos HP. An in vitro study, evaluating the effect of sunitinib and/or lapatinib on two glioma cell lines. Invest New Drugs, 2010; 28:554-60

Investigational New Drugs