PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR BETA-GLUCOSIDASE WITH TRANSGLYCOSYLATION AND EXO-GLUCOSIDASE ACTIVITIES FROM FUSARIUM-OXYSPORUM

 
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PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR BETA-GLUCOSIDASE WITH TRANSGLYCOSYLATION AND EXO-GLUCOSIDASE ACTIVITIES FROM FUSARIUM-OXYSPORUM (EN)

CHRISTAKOPOULOS, P (EN)
GOODENOUGH, PW (EN)
MACRIS, BJ (EN)
BHAT, MK (EN)
KEKOS, D (EN)
CLAEYSSENS, M (EN)

N/A (EN)

An extracellular beta-glucosidase from Fusarium oxysporum was purified to homogeneity by gel filtration and ion-exchange chromatographies. The enzyme, a monomeric protein of 110 kDa, was maximally active at pH 5.0-6.0 and at 60 degrees C. It hydrolysed 1-->4-linked aryl-beta-glucosides and 1-->4-linked, 1-->3-linked and 1-->6-linked beta-glucosides. The apparent K-m and k(cat) values for p-nitrophenyl beta-D-glucopyranoside (4-NpGlcp) and cellobiose were 0.093 (K-m), 1.07 mM (k(cat)) and 1802 (K-m), 461.5 min(-1) (k(cat)), respectively. Glucose and gluconolactone inhibited the enzyme competitively with K-i values of 2.05 mM and 3.03 mu M, respectively. Alcohols activated the enzyme; butanol showed maximum effect (2.2-fold at 0.5 M) while methanol increased the activity by 1.4-fold at 1 M. The enzyme catalysed the synthesis of methylglucosides, ethylglucoside and propylglucosides, as well as trisaccharides in the presence of different alcohols and disaccharides, respectively In addition, the enzyme hydrolysed the unsubstituted and methylumbelliferyl cello-oligosaccharides [MeUmb(Glc)(n)] but the rate of hydrolysis decreased with increasing chain length. Analysis of prod ucts released from MeUmb(Glc)(n) as a function of time revealed that the enzyme attacked these substrates in a stepwise manner and from both ends. Thus, beta-glucosidase from F. oxysporum, with the above interesting properties, could be of commercial interest. (EN)

journalArticle

ENZYMES (EN)
fusarium oxysporum (EN)
TRICHODERMA-KONINGII (EN)
FERMENTATION (EN)
ETHANOL (EN)
VANDERWALT (EN)
CELLULASE ACTIVITY (EN)
SPOROTRICHUM-THERMOPHILE (EN)

Εθνικό Μετσόβιο Πολυτεχνείο (EL)
National Technical University of Athens (EN)

EUROPEAN JOURNAL OF BIOCHEMISTRY (EN)

1994


SPRINGER VERLAG (EN)



*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.