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Purification and mode of action of an alkali-resistant endo-1,4-β- glucanase from Bacillus pumilus

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Purification and mode of action of an alkali-resistant endo-1,4-β- glucanase from Bacillus pumilus (EN)
Christakopoulos, P (EN)
Fountoukidis, G (EN)
Hatzinikolaou, DG (EN)
Macris, BJ (EN)
Claeyssens, M (EN)
Kekos, D (EN)
journalArticle (EN)
2014-03-01T01:15:05Z
1999 (EN)
Alkaline endo-1,4-beta-D-glucanase was secreted by Bacillus pumilus grown in submerged culture on a combination of oat spelt xylan and corn starch as carbon sources. The enzyme was purified to homogeneity by Sephacryl S-200 and Q-Sepharose column chromatography. The protein corresponded to molecular mass and pi values of 67 kDa and 3.7, respectively. The enzyme was optimally active at pH 7.0-8.0 and 60 degrees C and retained 50% of its optimum activity at pH 12. The most notable characteristic of the endoglucanase was its high stability up to pH 12 for 20 h at 30 degrees C. The enzyme hydrolyzed carboxymethylcellulose (CMC) and cello-oligosaccharides but was inactive on cellobiose, cellotriose, Avicel, xylan, 4-nitrophenyl-beta-D-glucoside, 4-nitrophenyl-beta-D-cellobioside, and 4-nitrophenyl-beta-D-xyloside. Analysis of reaction mixtures by HPLC revealed that the enzyme produced almost exclusively cellotriose when acted on CMC and appeared to hydrolyze cello-oligosaccharides by successively releasing cellotriose. The use of 4-methylumbelliferyl cello-oligosaccharides and the determination of bond cleavage frequency revealed that the enzyme preferentially hydrolyzed the third glycosidic bond adjacent to the glycon. The enzyme mediated a decrease in the viscosity of CMC associated with a release of only small amounts of reducing sugar. The enzyme activity was not inhibited by metal ions, surfactants, and chelating agents used as components of laundry detergents. (C) 1999 Academic Press. (EN)
Biochemistry & Molecular Biology (EN)
Biophysics (EN)
Chromatography, Ion Exchange (EN)
Molecular Weight (EN)
Metals (EN)
cellulase (EN)
Substrate Specificity (EN)
Glucose (EN)
Polysaccharides (EN)
enzyme purification (EN)
Cellobiose (EN)
enzyme activity (EN)
Bacillus (EN)
Triticum aestivum subsp. spelta (EN)
priority journal (EN)
Catalysis (EN)
Surface-Active Agents (EN)
enzyme analysis (EN)
Alkalies (EN)
Purification (EN)
Pumilus (EN)
Zea mays (EN)
Electrophoresis, Polyacrylamide Gel (EN)
Enzyme Stability (EN)
bacterial enzyme (EN)
article (EN)
Mode of action (EN)
enzyme stability (EN)
Cellulase (EN)
Bacteria (microorganisms) (EN)
Bacillus pumilus (EN)
nonhuman (EN)
Enzyme Activation (EN)
column chromatography (EN)
enzyme mechanism (EN)
Chromatography, Gel (EN)
Chelating Agents (EN)
Alkali resistant endoglucanase (EN)
Hydrogen-Ion Concentration (EN)
Archives of Biochemistry and Biophysics (EN)
English
ACADEMIC PRESS INC (EN)
National Technical University of Athens
Digital Library of National Technical University of Athens | Dspace@NTUA
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