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Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan

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Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan (EN)
Katapodis, P (EN)
Christakopoulos, P (EN)
Vardakou, M (EN)
Panayotou, G (EN)
Kekos, D (EN)
Samiotaki, M (EN)
journalArticle (EN)
2014-03-01T01:19:17Z
2003 (EN)
Microbial endo-beta-1,4-xylanases(EXs,EC3.2.1.8) belonging to glycanase families 10 and 11 differ in their action on water-unextractable arabinoxylan (WU-AX). WU-AX was incubated with different levels of a Thermoascus aurantiacus family 10 and a Sporotrichum thermophile family 11 endoxylanases. At 10 g l(-1) arabinoxylan, enzyme concentrations (K-E values) needed to obtain half-maximal hydrolysis rates (V-max values) were 4.4 nM for the xylanase from T aurantiacus and 7.1 nM for the xylanase from S. thermophile. Determination of V-max/K-E revealed that the family 10 enzyme hydrolysed two times more efficiently WU-AX than the family 11 enzyme. Molecular weights of the products formed were assessed and separation of feruloyl-oligosaccharides was achieved by anion-exchange and size-exclusion chromatography (SEC). The main difference between the feruloylated products by xylanases of family 10 and 11 concerned the length of the products containing feruloyl-arabinosyl substitution. The xylanase from T aurantiacus liberated from WU-AX a feruloyl arabinoxylodisaccharide (FAX(2)) as the shortest feruloylated fragment in contrast with the enzyme from S. thermophile, which liberated a feruloyl arabinoxylotrisaccharide (FAX(3)). These results indicated that different factors govern WU-AX breakdown by the two endoxylanases. (C) 2003 Elsevier B.V. All rights reserved. (EN)
Biochemistry & Molecular Biology (EN)
Water (EN)
unclassified drug (EN)
Chromatography, Ion Exchange (EN)
gel permeation chromatography (EN)
Eurotiales (EN)
Biochemistry (EN)
oligosaccharide (EN)
arabinoxylan (EN)
Sporotrichum (EN)
Enzyme family (EN)
Sporothrix (EN)
Thermoascus aurantiacus (EN)
disaccharide (EN)
Xylans (EN)
xylan endo 1,3 beta xylosidase (EN)
incubation time (EN)
molecular size (EN)
Thermoascus (EN)
Oligosaccharides (EN)
water (EN)
article (EN)
Mode of action (EN)
Hydrolysis (EN)
Corynascus heterothallicus (EN)
controlled study (EN)
feruloyl arabinoxydisaccharide (EN)
Endoxylanase (EN)
anion exchange (EN)
nonhuman (EN)
Chromatography, Gel (EN)
Endo-1,4-beta Xylanases (EN)
Spectrophotometry (EN)
hydrolysis (EN)
Coumaric Acids (EN)
Arabinoxylan (EN)
International Journal of Biological Macromolecules (EN)
English
ELSEVIER SCIENCE BV (EN)
National Technical University of Athens
Digital Library of National Technical University of Athens | Dspace@NTUA
Κεντρική Βιβλιοθήκη Ε.Μ.Π.
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