ENZYMATIC-SYNTHESIS OF TRISACCHARIDES AND ALKYL BETA-D-GLUCOSIDES BY THE TRANSGLYCOSYLATION REACTION OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM

This item is provided by the institution :
National Technical University of Athens

Repository :
Digital Library of National Technical University of Athens | Dspace@NTUA

see the original item page
in the repository's web site and access all digital files if the item^*

Title

ENZYMATIC-SYNTHESIS OF TRISACCHARIDES AND ALKYL BETA-D-GLUCOSIDES BY THE TRANSGLYCOSYLATION REACTION OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM (EN)

Creator

CHRISTAKOPOULOS, P (EN)

MACRIS, BJ (EN)

BHAT, MK (EN)

KEKOS, D (EN)

Type

journalArticle (EN)

Partial document
Publication in journal (EN)

Article
Scientific article (EN)

Issued

2014-03-01T01:42:48Z

1994 (EN)

Year

1994 (EN)

Description

Purified beta-glucosidase from Fusarium oxysporum catalyses hydrolysis and transglycosylation reactions. By utilizing the transglycosylation reaction, trisaccharides and alkyl beta-D-glucosides were synthesized under optimal conditions in the presence of various disaccharides and alcohols. The yields of trisaccharides and alkyl beta-D-glucosides were 22-37% and 10-33% of the total sugar, respectively. The enzyme retained 70-80% of its original activity in the presence of 25% (w/v) methanol, ethanol and propanol. Thus, beta-glucosidase from F. oxysporum appears to be an ideal enzyme for the synthesis of useful trisaccharides and alkyl beta-D-glucosides. (EN)

Scientific field

Biochemistry & Molecular Biology (EN)

Natural Sciences ▶ Biological sciences
Biochemistry and Molecular Biology (EN)

Subject

FUSARIUM OXYSPORUM (EN)

CATALYZED SYNTHESIS (EN)

LACTOSE (EN)

HYDROLYSIS (EN)

XYLOSIDASE (EN)

BETA-GLUCOSIDASE (EN)

TRANSGLYCOSYLATION (EN)

Journal