Factors affecting the specificity of beta-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-beta-D-glucosides

This item is provided by the institution :
National Technical University of Athens

Repository :
Digital Library of National Technical University of Athens | Dspace@NTUA

see the original item page
in the repository's web site and access all digital files if the item^*

Title

Factors affecting the specificity of beta-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-beta-D-glucosides (EN)

Creator

Christakopoulos, P (EN)

Tsitsimpikou, C (EN)

Macris, BJ (EN)

Makropoulou, M (EN)

Kolisis, FN (EN)

Kekos, D (EN)

Type

journalArticle (EN)

Partial document
Publication in journal (EN)

Article
Scientific article (EN)

Issued

2014-03-01T01:47:35Z

1998 (EN)

Year

1998 (EN)

Description

Fusarium oxysporum beta-glucosidase has been used to catalyze the production of alkyl-beta-D-glucosides from various disaccharides, based on the transglucosylation reaction, in the presence of primary, secondary and tertiary alcohols as glucosyl accepters. Primary alcohols were found to be the best accepters. The influence of the glucosyl donor concentration, as well as the enzyme specificity towards the cleaved glucosidic bond and the aglucone part of the donor, have also been investigated. The enzyme does not exhibit regiospecificity and seems to be unspecific towards the aglucone part. The specificity of the beta linkage has been confirmed by proton nuclear magnetic resonance (H-1 NMR) analysis. (C) 1998 Elsevier Science B.V. All rights reserved. (EN)

Scientific field

Biochemistry & Molecular Biology (EN)

Natural Sciences ▶ Biological sciences
Biochemistry and Molecular Biology (EN)

Subject

transglucosylation (EN)

Fusarium oxysporum (EN)

REVERSE HYDROLYSIS (EN)

PURIFICATION (EN)

ENDO-1,4-BETA-D-GLUCANASE (EN)

HYDROLYSIS REACTION (EN)

beta-glucosidase (EN)

Journal