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The AzgA purine transporter of Aspergillus nidulans - Characterization
of a protein belonging to a new phylogenetic cluster
Cecchetto, G
Amillis, S
Diallinas, G
Scazzocchio, C and
Drevet, C
The azgA gene of Aspergillus nidulans encodes a
hypoxanthine-adenine-guanine transporter. It has been cloned by a novel
transposon methodology. The null phenotype of azgA was defined by a
number of mutations, including a large deletion. In mycelia, the azgA
gene is, like other genes of purine catabolism, induced by uric acid and
repressed by ammonium. Its transcription depends on the pathway-specific
UaY zinc binuclear cluster protein and the broad domain AreA GATA
factor. AzgA is not closely related to any other characterized membrane
protein, but many close homologues of unknown function are present in
fungi, plants, and prokaryotes but not metazoa. Two of three data bases
and the phylogeny presented in this article places proteins of this
family in a cluster clearly separated (but perhaps phylogenetically
related) from the NAT family that includes other eukaryotic and
prokaryotic nucleobase transporters. Thus AzgA is the first
characterized member of this family or subfamily of membrane proteins.
(EN)
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