Comparative study of biocompatibility among different dialysis membranes using monocytes activation with flow cytometry measurement (CD 14+, CD 16+), burst activation and monocyte phagocytosis

Μελέτη της βιοσυμβατότητας μεμβρανών αιμοκάθαρσης μέσω της κινητικής των μονοκυττάρων με κυτταρομετρία ροής (CD 14+, CD 16+) και της δοκιμασίας ελέγχου αναπνευστικής έκρηξης και φαγοκυτταρικής λειτουργίας

Comparative study of biocompatibility among different dialysis membranes using monocytes activation with flow cytometry measurement (CD 14+, CD 16+), burst activation and monocyte phagocytosis

Γριβέας, Ιωάννης

Griveas, Ioannis

PhD Thesis

2006

Since the early observation of a transient leukopenia in the first minutes of haemodialysis (HD) by Kaplow et al in 1968, this issue has undergone extensive investigation. Ten years later, Graddock et al correlated this transient leukopenia with complement activation during HD. Since then, most studies have focused on the pathophysiology of granulocytes during HD. However, it has been recently suggested that the HD process influences monocytes as well. Peripheral blood monocytes are not a homogenous population and can be subdivided into discrete subpopulations by virtue of their surface antigen expression. A minor subset of circulating monocytes express the FcA receptor III (CD 16) together with low levels of the lipopolysaccharide (LPS ) receptor antigen (CD 14). These cells are described as the CD14+ CD16+ monocyte subpopulation. In normal health subjects CD14+ CD16+ subset accounts for about 7-10% of all circulating blood monocytes. However, during infectious or inflammatory diseases CD14+ CD16+ monocyte numbers are markedly expanded. Blood-membrane contact leads to an increased cellular activation and sequestration into the capillary bed of the lung. On the other hand, patients with chronic renal failure have increased susceptibility to various pathogens, especially to bacterial infection. This has been related to alterations in immune response and to disturbances of polymorphonuclear leukocytes (PMN) and monocytes (MO), which include chemotaxis, adherence and phagocytic and bacterial activity, especially as the consequence of blood-membrane contact. During ingestion of microorganisms, PMN generate highly reactive oxygen species with increased oxygen consumption, the so-called ‘respiratory burst’. The influence of the sequestration on the number of mature monocytes was studied by analyzing the fate of monocytes and particularly of the CD14+CD16+ subpopulation during haemodialysis (HD) treatment. The present study was aimed at examining also the effects of blood-membrane contact on PMN and MO, with two dialysis membrane groups. In particular, we studied phagocytosis activation due to contact with the dialysis membrane during extracorporeal sessions. We also evaluated respiratory burst both in PMN and MO.

Κλινική Ιατρική

Ιατρική και Επιστήμες Υγείας

Βιοσυμβατότητα

Flow cytometry

Medical and Health Sciences

Μεμβράνες αιμοκάθαρσης

Κυτταρομετρία ροής

Ενεργοποίηση συμπληρώματος

Κλινική Ιατρική

Monocytes activation

Κινητική μονοκυττάρων

Κινητική πολυμορφοπυρήνων

Clinical Medicine

Complement activation

Φαγοκυτταρική λειτουργία

Burst activation

Granulocytes activation

Biocompatibility

Dialysis membranes

Phagocytosis

Ιατρική και Επιστήμες Υγείας

Αναπνευστική έκρηξη

Ελληνική γλώσσα

Democritus University of Thrace (DUTH)

Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ)

Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Τμήμα Ιατρικής. Τομεάς Γενικής Παθολογίας. Κλινική Νεφρολογίας Πανεπιστημιακού Γενικού Νοσοκομείου Αλεξανδρούπολης

Εθνικό Κέντρο Τεκμηρίωσης και Ηλεκτρονικού Περιεχομένου (ΕΚΤ)

Εθνικό Αρχείο Διδακτορικών Διατριβών

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