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ACTION SPECTRUM OF LIGHT-HARVESTING-II APOPROTEIN TRANSCRIPT ACCUMULATION AND COORDINATED TRANSLATION

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ΦΑΣΜΑ ΔΡΑΣΗΣ ΤΗΣ ΜΕΤΑΓΡΑΦΗΣ ΤΟΥ CAB ΓΟΝΙΔΙΟΥ ΚΑΙ ΤΟΥ ΠΡΟΙΟΝΤΟΣ ΤΟΥ ΚΑΤΑ ΤΗΝ ΣΤΑΘΕΡΟΠΟΙΗΣΗ ΤΟΥ ΣΤΟ ΘΥΛΑΚΟΕΙΔΕΣ
ACTION SPECTRUM OF LIGHT-HARVESTING-II APOPROTEIN TRANSCRIPT ACCUMULATION AND COORDINATED TRANSLATION
Τριανταφυλλόπουλος, Κωνσταντίνος
PhD Thesis
1994
IN AN ATTEMPT TO ASCERTAIN THAT THE PHYTOCHROME CHROMOPROTEINS DO INDEED CONSTITUTE THE PHOTORECEPTOR INVOLVED IN LHC-II-APOPROTEIN-TRANSCRIPT ACCUMULATION AND THE SYNCHRONIZATION OF THE ENDOGENOUS CIRCADIAN CLOCK, WE STUDIED THEACTION SPECTRUM OF CAB TRANSCRIPTION AND ITS REVERSAL BY FAR-RED LIGHT, IN 9-10-DAY-OLD ETIOLATED PHASEOLUS VULGARIS LEAVES. TRANSCRIPT ACCUMULATION WASDETECTED BY HYBRIDIZATION EXPERIMENTS OR IMMUNOPRECIPITATION OF THE IN VITRO TRANSLATION PRODUCTS, OBTAINED FROM ISOLATED LEAF POLY(A)-RICH MRNA SPECIES. IT WAS FOUND THAT THE ACCUMULATION OF THE PRECURSOR-LHC-II- APOPROTEIN MRNA DEPENDS HIGHLY ON THE WAVELENGTH OF LIGHT EXPOSURE, THE ACTION SPECTRUM OF ITS ACCUMULATION BEING CLOSELY CORRELATED WITH ABSORPTION SPECTRUM OF THE PR FORM; WE ALSO CONCLUDE THAT PROBABLY THE PFR FORM OF PHYTOCHROME MEDIATING CAB-GENE TRANSCRIPTION IS THAT OF TYPE I (PIFR). IN ADDITION, IMMUNOBLOT ANALYSIS OF TOTAL LDS-SOLUBILIZED PROTEIN EXTRACTS OF LEAVES PREVIOUSLY EXPOSED UNDER THE SAME LIGHT CONDITIONS, CLOSELY FOLLOWS THE PATTERN OF ITS TRANSCRIPT ACCUMULATION AND THE ACTION SPECTRUM OF PROTOCHLOROPHYLLIDE PHOTOCONVERSION. SIMILAR LIGHT-DEPENDANCE PATTERN SHOWS THE MATURE D1, REACTION CENTER, PROTEIN CLOSELY COORDINATED WITH THE MATURE LHC-II PROTEIN. ON THE CONTRARY, THE PSI REACTION CENTER PROTEIN (CPI) APPEARS INDEPENDANCE FROM THE LIGHT QUALITY.
Φυσικές Επιστήμες ➨ Βιολογία
Photosystem II (PSII)
Western blot
ΠΡΩΤΟΧΛΩΡΟΦΥΛΛΗ
ΦΑΣΜΑ ΔΡΑΣΗΣ
ΦΥΤΟΧΡΩΜΑ
CELL-FREE EXTRACT
ACTION SPECTRUM
REACTION CENTER PROTEIN CPI
ΑΝΟΣΟΚΑΘΙΖΗΣΗ
mRNA
ΓΟΝΙΔΙΟ CAB
IN VITRO TRANSLATION
Φυσικές Επιστήμες
ΜΕΤΑΦΡΑΣΤΙΚΟ ΣΥΣΤΗΜΑ
Μεταγραφή
Chlorophyll A
Northern blot
PROTOCHLOROPHYLL
CAB GENE EXPRESSION
DOT BLOT
ΠΡΩΤΕΙΝΗ D1
ΦΩΤΟΑΠΟΔΕΚΤΗΣ
Natural Sciences
Χλωροφύλλη Α
PHYTOCHROME
ΣΥΜΠΛΟΚΟ ΣΥΛΛΟΓΗΣ ΦΩΤΟΝΙΩΝ
ΚΕΝΤΡΟ ΑΝΤΙΔΡΑΣΗΣ
ΦΑΣΟΛΙ
LHC-II
LIGHT-HARVESTING
Biological Sciences
ΦΩΤΟΓΟΝΙΔΙΟ
REACTION CENTER PROTEIN D1
ΠΡΩΤΕΙΝΗ CPI
Μετάφραση
Immunoprcipitation
Βιολογία
Φωτοσύστημα ΙΙ
Beans
Phaseolus vulgaris
CAB GENE
ΥΒΡΙΣΜΟΣ
Photoreceptors
TRANSCRIPT
ΜΗΝΥΜΑ RNA
Greek
Πανεπιστήμιο Πατρών
University of Patras
Πανεπιστήμιο Πατρών. Σχολή Θετικών Επιστημών. Τμήμα Βιολογίας
National Documentation Centre (EKT)
National Archive of PhD Theses
Συλλογή ΕΑΔΔ



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