Μελέτη έκφρασης ορολογικών δεικτών σε ασθενείς με ΙΦΝΕ από την ΒΔ Ελλάδα και συσχέτιση με κλινικοεργαστηριακά δεδομένα και περιβαλλοντικούς παράγοντες

Study of expression of serological biomarkers in patients with IBD from NW Greece and association with clinical data and environmental factors

Μελέτη έκφρασης ορολογικών δεικτών σε ασθενείς με ΙΦΝΕ από την ΒΔ Ελλάδα και συσχέτιση με κλινικοεργαστηριακά δεδομένα και περιβαλλοντικούς παράγοντες

Kyriakidi, Kallirroi

Κυριακίδη, Καλλιρρόη

PhD Thesis

2019

Ulcerative colitis and Crohn's disease belong to the same group of Idiopathic Inflammatory Bowel Disease (IBD) with unknown aetiopathogenesis. IBD is a chronic disease with outbursts and recessions. Although symptoms are usually treated with appropriate treatment, they impair the daily activities of the person causing significant morbidity. According to epidemiological studies that have been conducted both internationally and in our area, the number of patients with IBD is constantly increasing, suggesting the existence of an unknown environmental agent that goes into everyday life and causes disease in genetically predisposed individuals. In recent years, research into the aetiopathogenesis of IBD has increased sharply as the problem is continually increasing and therapeutically complicated. Diagnosis and treatment of IBD is very difficult, and despite the various diagnostic tests and available pharmaceutical preparations, some patients are forced to undergo surgical removal of the bowel and other types of disability. For the above reasons, patients are monitored in specialized reference centers by specialized scientists. FThe purpose of this study is to study serological markers, their expression and their utility in the prevention, diagnosis and treatment of patients with IBD. More specifically, some known serological markers with proven specificity / sensitivity were studied in patient with IBD. In the present study, 116 biological samples of serum from patients with Crohn's disease (NK) and 92 biological serum samples from patients with ulcerative colitis (UC) were studied and 99 healthy controls (CTRLS) were also studied. Patients were diagnosed with IBD based on clinical, endoscopic, radiological and pathological criteria and voluntarily participated in this research, which was conducted at the Immunology Research Laboratory of the Medical School of the University of Ioannina in collaboration with the Gastroenterological Clinic of the General Hospital of Ioannina. The samples came from populations in Northwest Greece. The study concerned the existence of ANCA antibodies by the immunofluorescence technique using a kit with ethanol, in particular the separation of pANCA and cANCA antibodies. We also studied the presence of ASCA antibodies using a kit using the ELISA technique. We studied all patients and healthy controls for the presence of ANCA and ASCA antibodies. The results of the study enriched one of the largest databases of patients with IBD, in addition the results of the serological markers were combined with other clinical data from the database by the same patients and healthy controls. This objective was achieved as the Immunology Research Laboratory is one of the largest centers of study and recording of IBD on a pan-Hellenic scale. The study, recording and processing of the data was done using appropriate research statistical methods and modern data analysis of scientific data.

Ιατρική και Επιστήμες Υγείας ➨ Κλινική Ιατρική

Clinical Medicine

Medical and Health Sciences

ΙΦΝΕ

Biomarkers

IBD

Crohn's disease

Κλινική Ιατρική

Βιοδείκτες

Νόσος Crohn

Ιατρική και Επιστήμες Υγείας

Ελκώδης κολίτιδα

Ulcerative colitis

Ελληνική γλώσσα

Πανεπιστήμιο Ιωαννίνων

University of Ioannina

Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής. Τομέας Παθολογίας. Κλινική Γαστρεντερολογική

National Documentation Centre (EKT)

National Archive of PhD Theses

Συλλογή ΕΑΔΔ