δείτε την πρωτότυπη σελίδα τεκμηρίου στον ιστότοπο του αποθετηρίου του φορέα για περισσότερες πληροφορίες και για να δείτε όλα τα ψηφιακά αρχεία του τεκμηρίου*
Evaluation of apoptosis in varicose vein disease complicated by
superficial vein thrombosis
Filis, Konstantinos
Kavantzas, Nikolaos
Dalainas, Ilias and
Galyfos, George
Karanikola, Evridiki
Toutouzas, Konstantinos and
Tsioufis, Constantinos
Sigala, Fragiska
Background: The factors contributing to superficial vein thrombosis
(SVT) in patients with varicose vein disease are unclear. Differences in
vein wall apoptotic activity could be associated with the pathogenesis
of SVT. The aim of the study is to address the role of the programmed
cell death in the vein wall by comparing varicose veins with history of
SVT to uncomplicated varicose veins.
Patients and methods: Vein segments from the proximal part of the great
saphenous vein (GSV), the distal part of the vein and from a varicose
tributary, from 16 patients with varicose vein disease and one episode
of SVT, were evaluated for the immunohistochemical expression of
pro-apoptotic (Bax, p53, Caspase 3, BCL-6, BCL-xs), anti-apoptotic
(BCL-xl and BCL-2) and proliferation (Ki-67) markers. The results of
this study were compared to the results from the evaluation of 19
patients suffering from uncomplicated varicose vein disease and 10
healthy GSVs as controls.
Results: Overall, there was increased apoptosis in the distal part of
GSV compared to the proximal part documented by increased expression of
Box (p < 0.01), Caspase 3 (p = 0.01), BCL-xs (p < 0.01). The comparisons
of the markers’ expression between patients with varicose veins and
patients with a history of SVT showed significant differences among the
three different anatomic locations. In the proximal GSV, only BCL-xs was
higher in patients with SVT (p = 0.029). In the tributaries, Box, BCL-xl
and Ki-67 were higher in patients with SVT (p < 0.01). In the distal
GSV, increased Box, BCL-xs, BCL-xl and Ki-67 staining was observed in
the thrombosis group compared to uncomplicated veins (p < 0.01).
Conclusions: The vein wall in SVT shows increased pro-apoptotic activity
compared to uncomplicated disease and normal veins. Whether increased
vein wall cell apoptosis is a causative factor for SVT in varicose veins
disease or a repairing mechanism of the thrombosis itself needs further
research.
(EN)
*Η εύρυθμη και αδιάλειπτη λειτουργία των διαδικτυακών διευθύνσεων των συλλογών (ψηφιακό αρχείο, καρτέλα τεκμηρίου στο αποθετήριο) είναι αποκλειστική ευθύνη των αντίστοιχων Φορέων περιεχομένου.
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